Hotspot

trnT-GGU-psbD

LSC · 31660-32956 · 1297 bp

Back to Chrysosplenium

Pi 0.0419

MarkerSeek score 81.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1297
variable_sites: 298
pi: 0.0419
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	TCTCCGTAACCAGTCATCCA	50.0	50.0	57.9	57.8	1260-1670 bp	1.0	89.4
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	TCTCCGTAACCAGTCATCCA	50.0	50.0	58.3	57.8	1223-1633 bp	1.0	87.9
trnT-GGU-psbD_p3	GCCCTTTTAACTCAGCGGTA	GGTCTCTCCGTAACCAGTCA	50.0	55.0	57.9	58.5	1264-1674 bp	0.969697	86.3
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	GGTCTCTCCGTAACCAGTCA	50.0	55.0	58.3	58.5	1227-1637 bp	0.969697	84.9
trnT-GGU-psbD_p5	GCCATGGTAAGGCGTAAGTC	TCTCCGTAACCAGTCATCCA	55.0	50.0	58.7	57.8	1233-1643 bp	1.0	84.6