Hotspot

trnE-UUC-trnT-GGU

LSC · 30764-31587 · 824 bp

Back to Chrysosplenium

Pi 0.0666

MarkerSeek score 82.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 824
variable_sites: 234
pi: 0.0666
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	386-968 bp	1.0	89.8
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	365-947 bp	1.0	89.7
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	ACAACCGAAAGCCCCTTATC	55.0	50.0	58.3	57.9	412-994 bp	1.0	88.9
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.3	57.9	353-933 bp	0.969697	87.9
trnE-UUC-trnT-GGU_p5	TGTCCTGAACCACTAGACGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.7	57.9	332-912 bp	0.969697	87.7