Hotspot

rpoB-trnC-GCA

LSC · 26494-27562 · 1069 bp

Back to Chrysosplenium

Pi 0.043

MarkerSeek score 78.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1069
variable_sites: 259
pi: 0.043
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCCACCTACACAAGCAAAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1301-1991 bp	0.969697	89.5
rpoB-trnC-GCA_p2	CCCCACCTACACAAGCAAAT	CAATTAGATTCCCCGTCCGG	50.0	55.0	58.1	58.1	1445-2135 bp	0.757576	80.7
rpoB-trnC-GCA_p3	CCCCACCTACACAAGCAAAT	CCAATTAGATTCCCCGTCCG	50.0	55.0	58.1	58.1	1446-2091 bp	0.606061	74.7
rpoB-trnC-GCA_p4	TCTTGCATATCCCTACCGGT	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1382-1527 bp	0.454545	68.7
rpoB-trnC-GCA_p5	TCTTGCATATCCCTACCGGT	CAATTAGATTCCCCGTCCGG	50.0	55.0	57.9	58.1	1527-1671 bp	0.393939	65.2