Hotspot

rps16-trnQ-UUG

LSC · 6079-6828 · 750 bp

Back to Chrysosplenium

Pi 0.101

MarkerSeek score 83.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 750
variable_sites: 301
pi: 0.101
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CAGCGGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	435-1269 bp	1.0	91.2
rps16-trnQ-UUG_p2	GCGGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	433-1267 bp	1.0	91.2
rps16-trnQ-UUG_p3	AGCGGATCATGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	434-1268 bp	1.0	88.8
rps16-trnQ-UUG_p4	GCGTTGCTTTCTACCACATC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	410-1244 bp	0.969697	86.0
rps16-trnQ-UUG_p5	ATCATGTCCTTCAAGTCGCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	835-1263 bp	0.060606	51.0