Hotspot

trnK-UUU-rps16

LSC · 4267-4988 · 722 bp

Back to Chrysosplenium

Pi 0.0606
MarkerSeek score 80.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
722
variable_sites
231
pi
0.0606
unique_haplotype_count
31
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CAGCCCAATCGCTCTTTTTG GGTCAAAAGTGTAACACGCG 50.0 50.0 58.0 58.0 1811-2104 bp 0.878788 86.9
trnK-UUU-rps16_p2 CAGCCCAATCGCTCTTTTTG GTCAAAAGTGTAACACGCGG 50.0 50.0 58.0 58.0 1810-2103 bp 0.878788 86.9
trnK-UUU-rps16_p3 CAGCCCAATCGCTCTTTTTG CGGTCAAAAGTGTAACACGC 50.0 50.0 58.0 58.0 1812-2105 bp 0.818182 84.5
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG GGTCAAAAGTGTAACACGCG 55.0 50.0 58.0 58.0 1408-1694 bp 0.757576 82.1
trnK-UUU-rps16_p5 CCTTTCAGGATCAGTCGTGG GTCAAAAGTGTAACACGCGG 55.0 50.0 58.0 58.0 1407-1693 bp 0.757576 82.1