Hotspot

ndhA

SSC · 114643-116769 · 2127 bp

Back to Chrysopogon

Pi 0.00897

MarkerSeek score 53.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2127
variable_sites: 34
pi: 0.00897
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CCCAGTTAGCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	50.0	50.0	56.6	57.9	2245-2268 bp	1.0	69.9
ndhA_p2	ACCCAGTTAGCATAGGGAAC	ATGGGGGAAGTTGATCGTTG	50.0	50.0	56.6	57.9	2246-2269 bp	1.0	69.9
ndhA_p3	ACCCAGTTAGCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	47.6	50.0	58.4	57.9	2246-2269 bp	1.0	69.6
ndhA_p4	CCCAGTTAGCATAGGGAACA	GGGGGAAGTTGATCGTTGAA	50.0	50.0	56.6	57.8	2243-2266 bp	1.0	69.4
ndhA_p5	ACCCAGTTAGCATAGGGAAC	GGGGGAAGTTGATCGTTGAA	50.0	50.0	56.6	57.8	2244-2267 bp	1.0	69.4