Hotspot

ccsA

SSC · 109287-110252 · 966 bp

Back to Chrysopogon

Pi 0.00725

MarkerSeek score 53.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 14
pi: 0.00725
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CTCAAGCATCTCGGTTCGAA	TCAAAGGGTTCTCAAATCAAACA	50.0	34.8	58.0	56.8	1135-1136 bp	1.0	47.5
ccsA_p2	AAGCATCTCGGTTCGAATCC	TCAAAGGGTTCTCAAATCAAACA	50.0	34.8	58.1	56.8	1132-1133 bp	1.0	47.1
ccsA_p3	CTCAAGCATCTCGGTTCGAA	TCAAAGGGTTCTCAAATCAAACAA	50.0	33.3	58.0	57.5	1135-1136 bp	1.0	44.7
ccsA_p4	CTCAAGCATCTCGGTTCGAA	TTCAAAGGGTTCTCAAATCAAACA	50.0	33.3	58.0	57.5	1136-1137 bp	1.0	44.7
ccsA_p5	AAGCATCTCGGTTCGAATCC	TCAAAGGGTTCTCAAATCAAACAA	50.0	33.3	58.1	57.5	1132-1133 bp	1.0	44.3