Hotspot

atpF

LSC · 35247-36629 · 1383 bp

Back to Chrysopogon

Pi 0.00566

MarkerSeek score 62.30

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1383
variable_sites: 15
pi: 0.00566
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACGAGCGAAGTAAGTGATCG	TCGAAGGGTTGCCATTAGTG	50.0	50.0	57.8	57.9	1552-1557 bp	1.0	78.6
atpF_p2	ACGAGCGAAGTAAGTGATCG	CGAAGGGTTGCCATTAGTGT	50.0	50.0	57.8	58.2	1551-1556 bp	1.0	78.0
atpF_p3	AAGGACGAGCGAAGTAAGTG	TCGAAGGGTTGCCATTAGTG	50.0	50.0	57.7	57.9	1556-1561 bp	1.0	77.2
atpF_p4	AAGGACGAGCGAAGTAAGTG	CGAAGGGTTGCCATTAGTGT	50.0	50.0	57.7	58.2	1555-1560 bp	1.0	76.6
atpF_p5	CGAGCGAAGTAAGTGATCGA	TCGAAGGGTTGCCATTAGTG	50.0	50.0	57.5	57.9	1551-1556 bp	1.0	76.3