Hotspot

rpl32-trnL-UAG

SSC · 108602-109129 · 528 bp

Back to Chrysopogon

Pi 0.0224
MarkerSeek score 64.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
528
variable_sites
21
pi
0.0224
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTTTTTCTGGGCAACAAGC TTCGAACCGAGATGCTTGAG 50.0 50.0 58.4 58.0 613-625 bp 1.0 81.0
rpl32-trnL-UAG_p2 GGTTTTTCTGGGCAACAAGC GGATTCGAACCGAGATGCTT 50.0 50.0 58.4 58.1 616-628 bp 1.0 80.5
rpl32-trnL-UAG_p3 GGTTTTTCTGGGCAACAAGC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.4 57.9 586-598 bp 1.0 80.2
rpl32-trnL-UAG_p4 GGTTTTTCTGGGCAACAAGC AGCAGCGTGTCTACCAATTT 50.0 45.0 58.4 57.8 579-591 bp 1.0 79.8
rpl32-trnL-UAG_p5 GGTTTTTCTGGGCAACAAGC GCTTGAGCACTGCTTCCTAA 50.0 50.0 58.4 58.2 600-612 bp 1.0 79.6