Hotspot

ndhF-rpl32

SSC · 107601-108421 · 821 bp

Back to Chrysopogon

Pi 0.0116
MarkerSeek score 61.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
821
variable_sites
18
pi
0.0116
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGGAGAAGAGGA TCCAAAGATTTTTACGAATACGCT 55.0 33.3 58.1 57.3 934-960 bp 1.0 45.1
ndhF-rpl32_p2 CTGGAAGTGGGAGAAGAGGA TGACATCGAAGTACGTTTTT 55.0 35.0 58.1 53.2 906-932 bp 1.0 44.9
ndhF-rpl32_p3 CTGGAAGTGGGAGAAGAGGA TTGACATCGAAGTACGTTTT 55.0 35.0 58.1 53.2 907-933 bp 1.0 44.9
ndhF-rpl32_p4 CTGGAAGTGGGAGAAGAGGA TTTGACATCGAAGTACGTTT 55.0 35.0 58.1 53.2 908-934 bp 1.0 44.9
ndhF-rpl32_p5 CTGGAAGTGGGAGAAGAGGA TTTTGACATCGAAGTACGTT 55.0 35.0 58.1 53.2 909-935 bp 1.0 44.9