Hotspot

trnV-UAC-trnM-CAU

LSC · 54001-54214 · 214 bp

Back to Chrysopogon

Pi 0.021

MarkerSeek score 65.60

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 214
variable_sites: 7
pi: 0.021
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	AACGAGTTGCTCTACCGAAC	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.9	58.4	264-294 bp	1.0	82.2
trnV-UAC-trnM-CAU_p2	AACGAGTTGCTCTACCGAAC	CCGCCGTATGAAAGCAATAC	50.0	50.0	57.9	57.3	263-293 bp	1.0	80.2
trnV-UAC-trnM-CAU_p3	CGAGTTGCTCTACCGAACTG	CCCGCCGTATGAAAGCAATA	55.0	50.0	58.4	58.4	262-292 bp	1.0	79.6
trnV-UAC-trnM-CAU_p4	AACGAGTTGCTCTACCGAAC	TTTGAACCAATGACTCCCGC	50.0	50.0	57.9	58.8	279-309 bp	1.0	79.3
trnV-UAC-trnM-CAU_p5	AACGAGTTGCTCTACCGAAC	AGCGAAGTAAGTCCAATGCT	50.0	45.0	57.9	57.2	336-370 bp	1.0	78.9