Hotspot

ndhC-trnV-UAC

LSC · 52344-53322 · 979 bp

Back to Chrysopogon

Pi 0.00885
MarkerSeek score 57.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
979
variable_sites
16
pi
0.00885
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAACCGGGGCTAAAAGTCC GGTTCGAATCCGTATAGCCC 55.0 55.0 58.0 57.9 1084-1116 bp 1.0 82.5
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAAGTCC CGGTTCGAATCCGTATAGCC 55.0 55.0 58.0 58.3 1085-1117 bp 1.0 80.6
ndhC-trnV-UAC_p3 TAACCGGGGCTAAAAGTCCT GGTTCGAATCCGTATAGCCC 50.0 55.0 58.3 57.9 1083-1115 bp 1.0 80.0
ndhC-trnV-UAC_p4 CACTAACCGGGGCTAAAAGT GGTTCGAATCCGTATAGCCC 50.0 55.0 57.5 57.9 1086-1118 bp 1.0 78.8
ndhC-trnV-UAC_p5 TAACCGGGGCTAAAAGTCCT CGGTTCGAATCCGTATAGCC 50.0 55.0 58.3 58.3 1084-1116 bp 1.0 78.2