Hotspot

trnF-GAA-ndhJ

LSC · 50094-50674 · 581 bp

Back to Chrysopogon

Pi 0.01

MarkerSeek score 58.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 581
variable_sites: 11
pi: 0.01
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ACGTAAGGACTATATAACCCCCA	50.0	43.5	58.1	57.9	645-678 bp	1.0	58.9
trnF-GAA-ndhJ_p2	ATGCACAAGAAAGGGTCAGG	ACGTAAGGACTATATAACCCCCA	50.0	43.5	58.1	57.9	698-731 bp	1.0	58.4
trnF-GAA-ndhJ_p3	GCACAAGAAAGGGTCAGGAT	ACGTAAGGACTATATAACCCCCA	50.0	43.5	57.8	57.9	696-729 bp	1.0	57.4
trnF-GAA-ndhJ_p4	AATCCTCGTGTCACCAGTTC	ACGTAAGGACTATATAACCCCCA	50.0	43.5	57.5	57.9	648-681 bp	1.0	55.7
trnF-GAA-ndhJ_p5	AGGGTCAGGATAGCTCAGTT	ACGTAAGGACTATATAACCCCCA	50.0	43.5	57.4	57.9	687-720 bp	1.0	54.6