Hotspot

rps4-trnT-UGU

LSC · 47920-48238 · 319 bp

Back to Chrysopogon

Pi 0.0047

MarkerSeek score 60.90

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 319
variable_sites: 3
pi: 0.0047
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CTCCCAGACGGCGTATTTTT	CGAGGGTCATCGGTTCAAAT	50.0	50.0	58.3	58.0	383-401 bp	1.0	82.0
rps4-trnT-UGU_p2	CTCCCAGACGGCGTATTTTT	TTGTAATGCGAGGGTCATCG	50.0	50.0	58.3	58.1	391-409 bp	1.0	81.6
rps4-trnT-UGU_p3	CTCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	413-431 bp	1.0	80.8
rps4-trnT-UGU_p4	GTGAGTCCTGGTAAAGCTCC	CGAGGGTCATCGGTTCAAAT	55.0	50.0	57.6	58.0	399-417 bp	1.0	80.8
rps4-trnT-UGU_p5	GTGAGTCCTGGTAAAGCTCC	TTGTAATGCGAGGGTCATCG	55.0	50.0	57.6	58.1	407-425 bp	1.0	80.4