Hotspot

trnC-GCA-rpoB

LSC · 20404-21610 · 1207 bp

Back to Chrysopogon

Pi 0.0106

MarkerSeek score 65.30

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1207
variable_sites: 22
pi: 0.0106
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CTTCTCAAGTTCTTCCGCCA	50.0	50.0	58.1	57.8	1337-1377 bp	1.0	82.2
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TCTCAAGTTCTTCCGCCAAG	50.0	50.0	58.1	57.8	1335-1375 bp	1.0	82.2
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	ACTTCTCAAGTTCTTCCGCC	50.0	50.0	58.1	57.8	1338-1378 bp	1.0	82.2
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	TTCCGCCAAGCCTTGATTAA	50.0	45.0	58.1	57.8	1325-1365 bp	1.0	81.9
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	CTTCCGCCAAGCCTTGATTA	50.0	50.0	58.1	58.2	1326-1366 bp	1.0	81.6