Hotspot

trnG-UCC-trnT-GGU

LSC · 14250-16207 · 1958 bp

Back to Chrysopogon

Pi 0.0114
MarkerSeek score 59.80
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1958
variable_sites
42
pi
0.0114
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT ACCACTAAGTGAAAAGCCCT 38.1 45.0 55.5 56.7 2074-2117 bp 1.0 43.5
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 2044-2087 bp 1.0 43.3
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 2040-2083 bp 1.0 42.6
trnG-UCC-trnT-GGU_p4 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 2041-2084 bp 1.0 42.6
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT ACCACTAAGTGAAAAGCCCT 39.1 45.0 57.3 56.7 2076-2119 bp 1.0 42.5