Hotspot

ycf1

SSC · 130221-135968 · 5748 bp

Back to Chrysojasminum

Pi 0.00968

MarkerSeek score 46.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5748
variable_sites: 104
pi: 0.00968
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTCTCGATCGCTCTCATCCT	AAGATGGGGAAGATGGGGAA	50.0	50.0	58.0	58.0	1544-1670 bp	1.0	84.1
ycf1_p2	TTCTCGATCGCTCTCATCCT	ATGGGGAAGATGGGGAAGAA	50.0	50.0	58.0	58.0	1541-1667 bp	1.0	84.1
ycf1_p3	GTCCTTGAAGCAGGTTCCAA	AAGATGGGGAAGATGGGGAA	50.0	50.0	58.0	58.0	1278-1404 bp	1.0	82.7
ycf1_p4	GTCCTTGAAGCAGGTTCCAA	ATGGGGAAGATGGGGAAGAA	50.0	50.0	58.0	58.0	1275-1401 bp	1.0	82.7
ycf1_p5	GTCCTTGAAGCAGGTTCCAA	TTGGGGAAGATGGGGAAGAT	50.0	50.0	58.0	58.0	1284-1410 bp	1.0	82.6