Hotspot

ycf1-ndhF

SSC · 116876-116915 · 40 bp

Back to Chrysojasminum

Pi 0.0833

MarkerSeek score 74.10

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 40
variable_sites: 5
pi: 0.0833
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	AAGATGGGGAAGATGGGGAA	CGAATGGGGTTGGTGTTTTG	50.0	50.0	58.0	57.9	774-869 bp	1.0	84.2
ycf1-ndhF_p2	ATGGGGAAGATGGGGAAGAA	CGAATGGGGTTGGTGTTTTG	50.0	50.0	58.0	57.9	771-866 bp	1.0	84.2
ycf1-ndhF_p3	TTGGGGAAGATGGGGAAGAT	CGAATGGGGTTGGTGTTTTG	50.0	50.0	58.0	57.9	780-875 bp	1.0	84.2
ycf1-ndhF_p4	AAGATGGGGAAGATGGGGAA	AATATGTAGGGGGTGGACGT	50.0	50.0	58.0	57.8	797 bp	0.25	48.5
ycf1-ndhF_p5	ATGGGGAAGATGGGGAAGAA	AATATGTAGGGGGTGGACGT	50.0	50.0	58.0	57.8	794 bp	0.25	48.5