Hotspot

petA-psbJ

LSC · 69080-70169 · 1090 bp

Back to Chrysojasminum

Pi 0.011

MarkerSeek score 61.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1090
variable_sites: 24
pi: 0.011
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1136-1153 bp	1.0	80.7
petA-psbJ_p2	AGAAGGTTCAATTGGCCGAA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1135-1152 bp	1.0	79.8
petA-psbJ_p3	TGAGAAGGTTCAATTGGCCG	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.5	57.6	1137-1154 bp	1.0	78.4
petA-psbJ_p4	GAGAAGGTTCAATTGGCCGA	CTTGTGATCGGTTTAGTGGGT	50.0	47.6	58.2	57.9	1176-1193 bp	1.0	75.0
petA-psbJ_p5	AGAAGGTTCAATTGGCCGAA	CTTGTGATCGGTTTAGTGGGT	45.0	47.6	57.7	57.9	1175-1192 bp	1.0	74.1