Hotspot

pafI-cemA

LSC · 66196-67188 · 993 bp

Back to Chrysojasminum

Pi 0.0191

MarkerSeek score 59.90

Primer available yes

Label pafI-cemA

Feature Metadata

label_name: pafI-cemA
feature_type: igs
region: LSC
length_bp: 993
variable_sites: 35
pi: 0.0191
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI-cemA_p1	GGAAACAGGCTATAGCGCTT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	1146-1211 bp	1.0	83.1
pafI-cemA_p2	GGAAACAGGCTATAGCGCTT	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.0	58.0	1497-1562 bp	1.0	82.5
pafI-cemA_p3	CTAAAGTCTTGAGCAGCGGT	GAGAAATCCACCAGGGCAAA	50.0	50.0	57.9	58.1	1624-1688 bp	1.0	81.6
pafI-cemA_p4	ACTAAAGTCTTGAGCAGCGG	GGAATTCCTGAGCCCAAGAG	50.0	55.0	57.9	58.0	1976-2040 bp	1.0	81.4
pafI-cemA_p5	CTAAAGTCTTGAGCAGCGGT	GGAATTCCTGAGCCCAAGAG	50.0	55.0	57.9	58.0	1975-2039 bp	1.0	81.4