Hotspot

accD-trnM-CAU

LSC · 47332-48584 · 1253 bp

Back to Chrysojasminum

Pi 0.0191

MarkerSeek score 62.50

Primer available yes

Label accD-trnM-CAU

Feature Metadata

label_name: accD-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1253
variable_sites: 45
pi: 0.0191
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-trnM-CAU_p1	CCAATGGCTACGGGAATACC	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.1	58.0	1371-1547 bp	1.0	83.2
accD-trnM-CAU_p2	CCCAATGGCTACGGGAATAC	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.1	58.0	1372-1548 bp	1.0	83.2
accD-trnM-CAU_p3	TACCCGTTTGAACAGCATCC	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.2	58.0	1337-1513 bp	1.0	82.7
accD-trnM-CAU_p4	GGCTACGGGAATACCACCTA	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.3	58.0	1366-1542 bp	1.0	81.8
accD-trnM-CAU_p5	CATAACCCCAATGGCTACGG	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.4	58.0	1378-1554 bp	1.0	80.9