Hotspot

psaA-pafII

LSC · 44412-45253 · 842 bp

Back to Chrysojasminum

Pi 0.00567

MarkerSeek score 64.30

Primer available yes

Label psaA-pafII

Feature Metadata

label_name: psaA-pafII
feature_type: igs
region: LSC
length_bp: 842
variable_sites: 7
pi: 0.00567
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafII_p1	GCCCATTCCTCGAAAGAAGT	CATTCCCTTGACCCGTACTG	50.0	55.0	57.9	58.0	1003-1025 bp	1.0	81.6
psaA-pafII_p2	GCCCATTCCTCGAAAGAAGT	ATTCCCTTGACCCGTACTGA	50.0	50.0	57.9	58.1	1002-1024 bp	1.0	81.4
psaA-pafII_p3	GCCCATTCCTCGAAAGAAGT	TTCCCTTGACCCGTACTGAT	50.0	50.0	57.9	58.1	1001-1023 bp	1.0	81.4
psaA-pafII_p4	GCCCATTCCTCGAAAGAAGT	CTTGCGCGTACCAATTGAAG	50.0	50.0	57.9	58.1	928-950 bp	1.0	81.0
psaA-pafII_p5	GCCCATTCCTCGAAAGAAGT	GAACAAAAAGCCGCCGAATT	50.0	45.0	57.9	58.2	957-979 bp	1.0	80.1