Hotspot

trnT-GGU-psbD

LSC · 33999-35359 · 1361 bp

Back to Chrysojasminum

Pi 0.00451

MarkerSeek score 64.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1361
variable_sites: 12
pi: 0.00451
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1463-1498 bp	1.0	79.7
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1453-1488 bp	1.0	78.6
trnT-GGU-psbD_p3	ACTCAGGGGTAGAGTAACGC	GGACCAGCCTACAAAAACGA	55.0	50.0	58.5	58.1	1491-1526 bp	1.0	77.6
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	CAAAAACGAAACGGTCCCTC	50.0	50.0	58.3	57.6	1452-1487 bp	1.0	77.4
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	AAGAGCAATAGACCGGACCA	50.0	50.0	58.3	58.4	1477-1512 bp	1.0	77.1