Hotspot

rps16-trnQ-UUG

LSC · 6647-8439 · 1793 bp

Back to Chrysojasminum

Pi 0.0111

MarkerSeek score 67.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1793
variable_sites: 33
pi: 0.0111
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TGCTTTCTACCACAGCGTTT	TTTGGTCCCGCTATTCGAAG	45.0	50.0	58.0	58.0	1807-1872 bp	1.0	84.5
rps16-trnQ-UUG_p2	TTGCTTTCTACCACAGCGTT	TTTGGTCCCGCTATTCGAAG	45.0	50.0	58.0	58.0	1808-1873 bp	1.0	84.5
rps16-trnQ-UUG_p3	CAGATCCATCCACCAAGTCG	TTTGGTCCCGCTATTCGAAG	55.0	50.0	58.1	58.0	1832-1897 bp	1.0	84.3
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TTTGGTCCCGCTATTCGAAG	45.0	50.0	58.1	58.0	1818-1883 bp	1.0	83.7
rps16-trnQ-UUG_p5	CCACAACAGATCCATCCACC	TTTGGTCCCGCTATTCGAAG	55.0	50.0	58.2	58.0	1838-1903 bp	1.0	82.8