Hotspot

trg1

Genome · 7884-8603 · 720 bp

Back to Chrysochromulina

Pi 0.00139

MarkerSeek score 59.50

Primer available yes

Label trg1

Feature Metadata

label_name: trg1
feature_type: gene
region: Genome
length_bp: 720
variable_sites: 1
pi: 0.00139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trg1_p1	ATTTCTTCCGAAGCACCGAT	TATGCAAAGACTCGAGACGG	45.0	50.0	57.6	57.4	781 bp	1.0	72.4
trg1_p2	ACGTACCATTTTGCCCAGAT	TATGCAAAGACTCGAGACGG	45.0	50.0	57.5	57.4	855 bp	1.0	71.5
trg1_p3	TCTTCCGAAGCACCGATAAG	TATGCAAAGACTCGAGACGG	50.0	50.0	57.4	57.4	778 bp	1.0	71.0
trg1_p4	ATTTCTTCCGAAGCACCGAT	TTATGCAAAGACTCGAGACGG	45.0	47.6	57.6	58.1	782 bp	1.0	67.9
trg1_p5	TTCTTCCGAAGCACCGATAA	TATGCAAAGACTCGAGACGG	45.0	50.0	57.0	57.4	779 bp	1.0	67.3