Hotspot

rpoB

Genome · 8736-12071 · 3336 bp

Back to Chrysochromulina

Pi 0.000899

MarkerSeek score 55.60

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: Genome
length_bp: 3336
variable_sites: 3
pi: 0.000899
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	TACCACTGCTATACCCGGAG	TACCAACTAGAGGCCTCTGG	55.0	55.0	58.0	57.8	228 bp	1.0	79.7
rpoB_p2	ACGTCAATCTGTACCACTGC	TACCAACTAGAGGCCTCTGG	50.0	55.0	57.9	57.8	239 bp	1.0	79.1
rpoB_p3	CTTAGAACACGATGACGCGA	TACCAACTAGAGGCCTCTGG	50.0	55.0	58.1	57.8	287 bp	1.0	78.7
rpoB_p4	TCTTAGAACACGATGACGCG	TACCAACTAGAGGCCTCTGG	50.0	55.0	58.1	57.8	288 bp	1.0	78.7
rpoB_p5	TGGGCTCAAATATGCAACGT	TACCAACTAGAGGCCTCTGG	45.0	55.0	58.2	57.8	255 bp	1.0	78.3