Hotspot

psbB

Genome · 85109-86638 · 1530 bp

Back to Chrysochromulina

Pi 0.00131

MarkerSeek score 59.40

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: Genome
length_bp: 1530
variable_sites: 2
pi: 0.00131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	AGTCCCTATTAGCAGAAAAGTGT	AAACAGCGTAAGAAAGTCAA	39.1	35.0	57.2	53.4	1660 bp	1.0	40.1
psbB_p2	AGTCCCTATTAGCAGAAAAGTGT	AACAGCGTAAGAAAGTCAAA	39.1	35.0	57.2	53.4	1659 bp	1.0	40.1
psbB_p3	AGTCCCTATTAGCAGAAAAGTGT	ACAGCGTAAGAAAGTCAAAA	39.1	35.0	57.2	53.4	1658 bp	1.0	40.1
psbB_p4	AGTCCCTATTAGCAGAAAAGTGT	AACAGCGTAAGAAAGTCAAAA	39.1	33.3	57.2	54.3	1659 bp	1.0	40.1
psbB_p5	AGTCCCTATTAGCAGAAAAGTGT	ACAGCGTAAGAAAGTCAAAAA	39.1	33.3	57.2	54.3	1658 bp	1.0	40.1