Hotspot

psbA

Genome · 45077-46159 · 1083 bp

Back to Chrysochromulina

Pi 0.00369

MarkerSeek score 54.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: Genome
length_bp: 1083
variable_sites: 4
pi: 0.00369
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AAAAACAGAAGGCTTAGAGC	CCTGGGAGGTTTTCAAGACC	40.0	55.0	53.8	58.1	1186 bp	1.0	46.0
psbA_p2	GCTTAGAGCCTTCTGTTTTT	CCTGGGAGGTTTTCAAGACC	40.0	55.0	53.8	58.1	1175 bp	1.0	46.0
psbA_p3	AAAAACAGAAGGCTTAGAGC	CTGGGAGGTTTTCAAGACCA	40.0	50.0	53.8	57.3	1185 bp	1.0	41.4
psbA_p4	GCTTAGAGCCTTCTGTTTTT	CTGGGAGGTTTTCAAGACCA	40.0	50.0	53.8	57.3	1174 bp	1.0	41.4
psbA_p5	GCTTAGAGCCTTCTGTTTTT	TCCTGGGAGGTTTTCAAGAC	40.0	50.0	53.8	57.0	1176 bp	1.0	40.3