Hotspot

groEL

Genome · 76425-78029 · 1605 bp

Back to Chrysochromulina

Pi 0.00312

MarkerSeek score 45.40

Primer available yes

Label groEL

Feature Metadata

label_name: groEL
feature_type: gene
region: Genome
length_bp: 1605
variable_sites: 5
pi: 0.00312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
groEL_p1	GCGGGTGCTTGAAAAACTTT	GACTCATAAGCCCAAGGTCG	45.0	55.0	58.1	58.1	1991 bp	1.0	79.3
groEL_p2	ATTCTCCGGGTGCAAATTGT	GACTCATAAGCCCAAGGTCG	45.0	55.0	58.1	58.1	2029 bp	1.0	79.2
groEL_p3	GCGGGTGCTTGAAAAACTTT	GATTGGGTCAACTGGGACTC	45.0	55.0	58.1	57.9	2414 bp	1.0	78.8
groEL_p4	ATTCTCCGGGTGCAAATTGT	GATTGGGTCAACTGGGACTC	45.0	55.0	58.1	57.9	2452 bp	1.0	78.7
groEL_p5	ATTGCGGGTGCTTGAAAAAC	GACTCATAAGCCCAAGGTCG	45.0	55.0	58.1	58.1	1994 bp	1.0	78.7