Hotspot

ccsA

Genome · 43440-44393 · 954 bp

Back to Chrysochromulina

Pi 0.00105

MarkerSeek score 59.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: Genome
length_bp: 954
variable_sites: 1
pi: 0.00105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CTTTGCTCCTGGTTGTAGCA	TGAACAGGAAGAAGTAATCTAGACT	50.0	36.0	58.1	57.0	1116 bp	1.0	40.1
ccsA_p2	TCTTTGCTCCTGGTTGTAGC	TGAACAGGAAGAAGTAATCTAGACT	50.0	36.0	57.8	57.0	1117 bp	1.0	40.1
ccsA_p3	CTTTGCTCCTGGTTGTAGCA	AGGAAGAAGTAATCTAGACTAGACCT	50.0	38.5	58.1	57.9	1111 bp	1.0	40.1
ccsA_p4	TGGATCTTTGCTCCTGGTTG	TGAACAGGAAGAAGTAATCTAGACT	50.0	36.0	57.8	57.0	1121 bp	1.0	40.1
ccsA_p5	GGATCTTTGCTCCTGGTTGT	TGAACAGGAAGAAGTAATCTAGACT	50.0	36.0	57.8	57.0	1120 bp	1.0	40.1