Hotspot

ccs1

Genome · 3430-4737 · 1308 bp

Back to Chrysochromulina

Pi 0.00153

MarkerSeek score 59.60

Primer available yes

Label ccs1

Feature Metadata

label_name: ccs1
feature_type: gene
region: Genome
length_bp: 1308
variable_sites: 2
pi: 0.00153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccs1_p1	TCCACTGCTCTACCAACTGA	AGAGCATATTTTAACTACACGACT	50.0	33.3	58.0	56.0	1438 bp	1.0	40.1
ccs1_p2	TCCCGGCTTGTTTGCTTATT	AGAGCATATTTTAACTACACGACT	45.0	33.3	58.1	56.0	1414 bp	1.0	40.1
ccs1_p3	ATCCCGGCTTGTTTGCTTAT	AGAGCATATTTTAACTACACGACT	45.0	33.3	57.9	56.0	1415 bp	1.0	40.1
ccs1_p4	TCCACTGCTCTACCAACTGA	AGAGCATATTTTAACTACACGACTT	50.0	32.0	58.0	56.6	1438 bp	1.0	40.1
ccs1_p5	CCACTGCTCTACCAACTGAG	AGAGCATATTTTAACTACACGACT	55.0	33.3	57.6	56.0	1437 bp	1.0	40.1