Hotspot

atpI

Genome · 19670-20371 · 702 bp

Back to Chrysochromulina

Pi 0.00142

MarkerSeek score 59.60

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: Genome
length_bp: 702
variable_sites: 1
pi: 0.00142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	GCACTATTCACGCCGAACTA	TGTGCGACTTGTGGAAAAGA	50.0	45.0	58.1	58.0	1152 bp	1.0	79.2
atpI_p2	GCACTATTCACGCCGAACTA	AACAGAAGCAGCTGAAACGA	50.0	45.0	58.1	58.0	879 bp	1.0	79.1
atpI_p3	TGCTTATGACCCACGAGTTG	TGTGCGACTTGTGGAAAAGA	50.0	45.0	57.9	58.0	1810 bp	1.0	79.0
atpI_p4	GCTTATGACCCACGAGTTGT	TGTGCGACTTGTGGAAAAGA	50.0	45.0	57.9	58.0	1809 bp	1.0	79.0
atpI_p5	GCACTATTCACGCCGAACTA	TAACGGATTGCGCATTAGCT	50.0	45.0	58.1	58.1	1483 bp	1.0	79.0