Hotspot

trnW-trnS

Genome · 92853-92911 · 59 bp

Back to Chrysochromulina

Pi 0.0169

MarkerSeek score 59.90

Primer available yes

Label trnW-trnS

Feature Metadata

label_name: trnW-trnS
feature_type: igs
region: Genome
length_bp: 59
variable_sites: 1
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-trnS_p1	AGTTCGCGCTCTTAGTTCAG	ACCCGTGATAGGAGAGGATG	50.0	55.0	58.0	58.0	234 bp	1.0	80.2
trnW-trnS_p2	GGGATAGTTCGCGCTCTTAG	ACCCGTGATAGGAGAGGATG	55.0	55.0	57.9	58.0	239 bp	1.0	79.9
trnW-trnS_p3	GCGCTCTTAGTTCAGTTGGT	ACCCGTGATAGGAGAGGATG	50.0	55.0	58.2	58.0	229 bp	1.0	78.7
trnW-trnS_p4	GTTCGCGCTCTTAGTTCAGT	ACCCGTGATAGGAGAGGATG	50.0	55.0	58.3	58.0	233 bp	1.0	77.9
trnW-trnS_p5	TCGCGCTCTTAGTTCAGTTG	ACCCGTGATAGGAGAGGATG	50.0	55.0	58.3	58.0	231 bp	1.0	77.9