Hotspot

psbJ-trnE

Genome · 50198-50371 · 174 bp

Back to Chrysochromulina

Pi 0.0172

MarkerSeek score 62.40

Primer available yes

Label psbJ-trnE

Feature Metadata

label_name: psbJ-trnE
feature_type: igs
region: Genome
length_bp: 174
variable_sites: 3
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-trnE_p1	TCCTCTTTGGATGGTGGCTA	AGGCCTAGGACATTTCCCTT	50.0	50.0	58.0	58.0	332 bp	1.0	80.3
psbJ-trnE_p2	CCTCTTTGGATGGTGGCTAC	AGGCCTAGGACATTTCCCTT	55.0	50.0	58.0	58.0	331 bp	1.0	80.3
psbJ-trnE_p3	CTTGCCGCCCTAGGTTTATT	AGGCCTAGGACATTTCCCTT	50.0	50.0	57.9	58.0	301 bp	1.0	80.3
psbJ-trnE_p4	ATTCCTCTTTGGATGGTGGC	AGGCCTAGGACATTTCCCTT	50.0	50.0	57.9	58.0	334 bp	1.0	79.4
psbJ-trnE_p5	TCCTCTTTGGATGGTGGCTA	TTACTTGCCCCCATCGTCTA	50.0	50.0	58.0	58.1	353 bp	1.0	79.4