Hotspot

rpl32-ndhF

SSC · 122789-123781 · 993 bp

Back to Chrysanthemum

Pi 0.00459
MarkerSeek score 65.50
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
SSC
length_bp
993
variable_sites
15
pi
0.00459
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 ACAAAGCTTTTAATGCCGCC CTTTTTGTTGCCGTCGGAAA 45.0 45.0 57.9 57.8 1182-1196 bp 1.0 80.3
rpl32-ndhF_p2 AATGCCGCCCAATATCCTTT CTTTTTGTTGCCGTCGGAAA 45.0 45.0 57.9 57.8 1171-1185 bp 1.0 80.3
rpl32-ndhF_p3 ACAAAGCTTTTAATGCCGCC AAGACTTTTTGTTGCCGTCG 45.0 45.0 57.9 57.5 1186-1200 bp 1.0 78.2
rpl32-ndhF_p4 ACAAAGCTTTTAATGCCGCC ACGAAGACTTTTTGTTGCCG 45.0 45.0 57.9 57.5 1189-1203 bp 1.0 78.2
rpl32-ndhF_p5 ACAAAGCTTTTAATGCCGCC CGAAGACTTTTTGTTGCCGT 45.0 45.0 57.9 57.5 1188-1202 bp 1.0 78.2