Hotspot

psaC-ndhD

SSC · 118770-118883 · 114 bp

Back to Chrysanthemum

Pi 0.038

MarkerSeek score 66.10

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 114
variable_sites: 8
pi: 0.038
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	CGGCTGTAAGAGATGCGAAT	AGGAACCCGCAAATATTGGT	50.0	45.0	58.1	57.5	271-273 bp	1.0	77.6
psaC-ndhD_p2	GAGGATTGTGTCGGCTGTAA	AGGAACCCGCAAATATTGGT	50.0	45.0	57.6	57.5	282-284 bp	1.0	75.6
psaC-ndhD_p3	GGATTGTGTCGGCTGTAAGA	AGGAACCCGCAAATATTGGT	50.0	45.0	57.6	57.5	280-282 bp	1.0	75.6
psaC-ndhD_p4	AGGATTGTGTCGGCTGTAAG	AGGAACCCGCAAATATTGGT	50.0	45.0	57.6	57.5	281-283 bp	1.0	75.5
psaC-ndhD_p5	AGAGGATTGTGTCGGCTGTA	AGGAACCCGCAAATATTGGT	50.0	45.0	58.4	57.5	283-285 bp	1.0	75.1