Hotspot

ycf1-rps15

SSC · 110330-112627 · 2298 bp

Back to Chrysanthemum

Pi 0.00285

MarkerSeek score 57.20

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 2298
variable_sites: 28
pi: 0.00285
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	TGACTTCATTTCGCCTATGA	TGAAATTCAACGGATCCGCT	40.0	45.0	54.5	57.9	2386-2408 bp	1.0	52.7
ycf1-rps15_p2	TTGACTTCATTTCGCCTATGA	TGAAATTCAACGGATCCGCT	38.1	45.0	55.3	57.9	2387-2409 bp	1.0	51.4
ycf1-rps15_p3	ATTGACTTCATTTCGCCTATGA	TGAAATTCAACGGATCCGCT	36.4	45.0	55.9	57.9	2388-2410 bp	1.0	47.9
ycf1-rps15_p4	TTGACTTCATTTCGCCTATG	TGAAATTCAACGGATCCGCT	40.0	45.0	53.7	57.9	2387-2409 bp	1.0	46.5
ycf1-rps15_p5	AAATTGACTTCATTTCGCCT	TGAAATTCAACGGATCCGCT	35.0	45.0	53.8	57.9	2390-2412 bp	0.9	42.6