Hotspot

rpl14-rpl16

LSC · 79702-79819 · 118 bp

Back to Chrysanthemum

Pi 0.00798
MarkerSeek score 56.60
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
118
variable_sites
3
pi
0.00798
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 TCGACGATTGCTAGCTCCTA AATAGCAGCGTCCAAAATGC 50.0 45.0 58.0 57.4 253-263 bp 1.0 80.7
rpl14-rpl16_p2 ACACATCAATTCTCGAGCCC AATAGCAGCGTCCAAAATGC 50.0 45.0 58.0 57.4 223-233 bp 1.0 80.6
rpl14-rpl16_p3 TCGACGATTGCTAGCTCCTA GCGTCCAAAATGCCTATACG 50.0 50.0 58.0 57.3 246-256 bp 1.0 80.0
rpl14-rpl16_p4 ACACATCAATTCTCGAGCCC GCGTCCAAAATGCCTATACG 50.0 50.0 58.0 57.3 216-226 bp 1.0 79.9
rpl14-rpl16_p5 AACGTCACCAATACGAGCAT AATAGCAGCGTCCAAAATGC 45.0 45.0 57.6 57.4 274-284 bp 1.0 77.8