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psbE-petL

LSC · 63431-64694 · 1264 bp

Back to Chrysanthemum

Pi 0.00353

MarkerSeek score 63.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1264
variable_sites: 16
pi: 0.00353
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.9	58.0	1339-1438 bp	1.0	84.5
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.9	58.0	1341-1440 bp	1.0	84.5
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.8	58.0	1262-1361 bp	1.0	83.7
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.8	58.0	1264-1363 bp	1.0	83.7
psbE-petL_p5	ACGAATAACCAACCCGCAAT	GCCGCTAGTAGAAAACCGAA	45.0	50.0	58.2	58.0	1348-1447 bp	1.0	83.5