Hotspot

trnR-UCU-trnT-GGU

LSC · 29734-30916 · 1183 bp

Back to Chrysanthemum

Pi 0.00224

MarkerSeek score 47.60

Primer available yes

Label trnR-UCU-trnT-GGU

Feature Metadata

label_name: trnR-UCU-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1183
variable_sites: 8
pi: 0.00224
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnT-GGU_p1	AGAAGACCTCTGTCCTATCCA	TACGCCTTACCGTGGGATTA	47.6	50.0	56.9	58.2	1245-1260 bp	1.0	63.4
trnR-UCU-trnT-GGU_p2	AGAAGACCTCTGTCCTATCCA	GCCTTACCGTGGGATTACTC	47.6	55.0	56.9	57.8	1242-1257 bp	1.0	63.3
trnR-UCU-trnT-GGU_p3	AGAAGACCTCTGTCCTATCCA	ATTTGAACCGAGGGCTTACG	47.6	50.0	56.9	58.3	1261-1276 bp	1.0	63.0
trnR-UCU-trnT-GGU_p4	AGGTTTAGAAGACCTCTGTCCT	TACGCCTTACCGTGGGATTA	45.5	50.0	57.9	58.2	1251-1266 bp	1.0	62.7
trnR-UCU-trnT-GGU_p5	AGGTTTAGAAGACCTCTGTCCT	GCCTTACCGTGGGATTACTC	45.5	55.0	57.9	57.8	1248-1263 bp	1.0	62.6