Hotspot

ycf29

Genome · 135447-136139 · 693 bp

Back to Chroomonas

Pi 0.376

MarkerSeek score 80.60

Primer available yes

Label ycf29

Feature Metadata

label_name: ycf29
feature_type: gene
region: Genome
length_bp: 693
variable_sites: 256
pi: 0.376
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf29_p1	AGAGTGGCTTGACCTACACT	TTCTAAATCAGGCGGACGTG	50.0	50.0	58.0	58.0	1451 bp	0.5	59.8
ycf29_p2	AGAGTGGCTTGACCTACACT	TTGACTCCGTTATTCGCAGG	50.0	50.0	58.0	58.0	2081 bp	0.5	59.8
ycf29_p3	AGAGTGGCTTGACCTACACT	ACTCCGTTATTCGCAGGTTC	50.0	50.0	58.0	58.0	2078 bp	0.5	59.8
ycf29_p4	AGAGTGGCTTGACCTACACT	GACTCCGTTATTCGCAGGTT	50.0	50.0	58.0	58.0	2079 bp	0.5	59.8
ycf29_p5	AGAGTGGCTTGACCTACACT	AAAGTCCATGCCACAATGGT	50.0	45.0	58.0	58.0	2022 bp	0.5	59.8