Hotspot

rps3

Genome · 102986-103645 · 660 bp

Back to Chroomonas

Pi 0.228

MarkerSeek score 80.20

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: Genome
length_bp: 660
variable_sites: 148
pi: 0.228
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	ATCTGTGAGCACTACCCTGT	CCGCTCTTGCTGTAATGGAT	50.0	50.0	58.1	58.0	1036 bp	0.5	59.2
rps3_p2	TGAGCACTACCCTGTAGCTT	CCGCTCTTGCTGTAATGGAT	50.0	50.0	58.1	58.0	1031 bp	0.5	59.2
rps3_p3	ATCTGTGAGCACTACCCTGT	TCCAGAGCTTGTAACCCGTA	50.0	50.0	58.1	58.1	914 bp	0.5	59.0
rps3_p4	TGAGCACTACCCTGTAGCTT	TCCAGAGCTTGTAACCCGTA	50.0	50.0	58.1	58.1	909 bp	0.5	58.9
rps3_p5	ATCTGTGAGCACTACCCTGT	AACTACACGATTTCCACGGG	50.0	50.0	58.1	57.9	885 bp	0.5	58.8