Hotspot

rps2

Genome · 66242-66940 · 699 bp

Back to Chroomonas

Pi 0.201

MarkerSeek score 80.30

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: Genome
length_bp: 699
variable_sites: 139
pi: 0.201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TGGACAAGCAGCTATTTGCA	AGAAGATGGGGGAGACACAA	45.0	50.0	58.1	58.0	1755 bp	0.5	58.9
rps2_p2	TGGACAAGCAGCTATTTGCA	GAAGATGGGGGAGACACAAC	45.0	55.0	58.1	57.9	1754 bp	0.5	58.3
rps2_p3	TGGACAAGCAGCTATTTGCA	CGAAGGAAAAGCTGACTGGT	45.0	50.0	58.1	58.1	1542 bp	0.5	58.3
rps2_p4	TGGACAAGCAGCTATTTGCA	AAAGCTGACTGGTTACGTGG	45.0	50.0	58.1	58.1	1535 bp	0.5	58.3
rps2_p5	TGGACAAGCAGCTATTTGCA	CATTGCACGGAATTATGGGC	45.0	50.0	58.1	57.9	1335 bp	0.5	58.2