Hotspot

infB

Genome · 77835-80177 · 2343 bp

Back to Chroomonas

Pi 0.334

MarkerSeek score 78.90

Primer available yes

Label infB

Feature Metadata

label_name: infB
feature_type: gene
region: Genome
length_bp: 2343
variable_sites: 767
pi: 0.334
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infB_p1	TATCGTCGCAAAGCTTCTCC	TTTCTTGTTTACTCGCGGGT	50.0	45.0	58.1	57.8	3000 bp	0.5	57.4
infB_p2	TATCGTCGCAAAGCTTCTCC	TCTTGTTTACTCGCGGGTAA	50.0	45.0	58.1	56.9	2998 bp	0.5	50.5
infB_p3	TATCGTCGCAAAGCTTCTCC	TTCTTGTTTACTCGCGGGTA	50.0	45.0	58.1	56.9	2999 bp	0.5	50.5
infB_p4	CGTCGCAAAGCTTCTCCAAT	TTTCTTGTTTACTCGCGGGT	50.0	45.0	59.2	57.8	2997 bp	0.5	48.4
infB_p5	TATCGTCGCAAAGCTTCTCC	TTCTTGTTTACTCGCGGGTAA	50.0	42.9	58.1	57.6	2999 bp	0.5	48.3