Hotspot

ccsA

Genome · 16087-17064 · 978 bp

Back to Chroomonas

Pi 0.27

MarkerSeek score 81.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: Genome
length_bp: 978
variable_sites: 257
pi: 0.27
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AAGCGGAAACTGCTACACAA	AGGCTGGACAAATTGCTTCA	45.0	45.0	58.0	58.0	2163 bp	0.5	59.7
ccsA_p2	AGCGGAAACTGCTACACAAA	AGGCTGGACAAATTGCTTCA	45.0	45.0	58.0	58.0	2162 bp	0.5	59.7
ccsA_p3	AAATCGTCGTATGGCTGCAT	AGGCTGGACAAATTGCTTCA	45.0	45.0	58.0	58.0	2145 bp	0.5	59.6
ccsA_p4	AAGCGGAAACTGCTACACAA	TGAATGACATAGCGTCCTGC	45.0	50.0	58.0	58.1	1788 bp	0.5	59.2
ccsA_p5	AGCGGAAACTGCTACACAAA	TGAATGACATAGCGTCCTGC	45.0	50.0	58.0	58.1	1787 bp	0.5	59.2