Hotspot

rps4-rbcL

Genome · 40315-42067 · 1753 bp

Back to Chroomonas

Pi 0.401

MarkerSeek score 82.90

Primer available yes

Label rps4-rbcL

Feature Metadata

label_name: rps4-rbcL
feature_type: igs
region: Genome
length_bp: 1753
variable_sites: 659
pi: 0.401
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-rbcL_p1	CCTACTATTCCAGCCGCAAG	GCAGTAGAAGACTCACCTGC	55.0	55.0	58.1	58.0	2259 bp	0.5	58.9
rps4-rbcL_p2	ATGCAAAGCGAATCAAGGGA	GCAGTAGAAGACTCACCTGC	45.0	55.0	58.2	58.0	2351 bp	0.5	58.7
rps4-rbcL_p3	TGCAAAGCGAATCAAGGGAT	GCAGTAGAAGACTCACCTGC	45.0	55.0	58.2	58.0	2350 bp	0.5	58.7
rps4-rbcL_p4	CCTACTATTCCAGCCGCAAG	ACAACTGTCCATGTTGCAGT	55.0	45.0	58.1	57.9	2274 bp	0.5	58.5
rps4-rbcL_p5	GGAATGGCGCCTACTATTCC	GCAGTAGAAGACTCACCTGC	55.0	55.0	58.2	58.0	2268 bp	0.5	58.5