Hotspot

rbcR-rpl27

Genome · 29769-30846 · 1078 bp

Back to Chroomonas

Pi 0.397

MarkerSeek score 80.60

Primer available yes

Label rbcR-rpl27

Feature Metadata

label_name: rbcR-rpl27
feature_type: igs
region: Genome
length_bp: 1078
variable_sites: 357
pi: 0.397
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcR-rpl27_p1	TACTCGACGCATTTCTTGGG	GGGAATGTAAGTGTAGGGCG	50.0	55.0	58.0	58.1	2101 bp	0.5	59.6
rbcR-rpl27_p2	TACTCGACGCATTTCTTGGG	TGCGTCCGAAGAAAAAGACA	50.0	45.0	58.0	58.1	2004 bp	0.5	59.5
rbcR-rpl27_p3	TACTCGACGCATTTCTTGGG	CGGCGATGATACTGAAGGAG	50.0	55.0	58.0	57.9	2344 bp	0.5	59.5
rbcR-rpl27_p4	TACTCGACGCATTTCTTGGG	AGGAGGGATCCTTTGGGAAA	50.0	50.0	58.0	57.9	2329 bp	0.5	59.4
rbcR-rpl27_p5	GGTCTTCTTCATTGGGCCAA	GGGAATGTAAGTGTAGGGCG	50.0	55.0	58.1	58.1	1713 bp	0.5	59.0