Hotspot

minE-rpl32

Genome · 27195-28101 · 907 bp

Back to Chroomonas

Pi 0.4

MarkerSeek score 82.30

Primer available yes

Label minE-rpl32

Feature Metadata

label_name: minE-rpl32
feature_type: igs
region: Genome
length_bp: 907
variable_sites: 350
pi: 0.4
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
minE-rpl32_p1	TTGTATTAGCCCATGACCGC	CGTCTAGCAAACTGGAGCAA	50.0	50.0	58.0	58.2	1248 bp	0.5	58.1
minE-rpl32_p2	GCTTGTCGATTAGATGGGCA	CGTCTAGCAAACTGGAGCAA	50.0	50.0	58.0	58.2	1464 bp	0.5	58.0
minE-rpl32_p3	CCGCTCTACGTTAAATGCCT	CGTCTAGCAAACTGGAGCAA	50.0	50.0	58.1	58.2	1232 bp	0.5	57.9
minE-rpl32_p4	TAAAACGCTTGCGCAGTTTT	CGTCTAGCAAACTGGAGCAA	40.0	50.0	57.8	58.2	1400 bp	0.5	56.8
minE-rpl32_p5	TTAAAACGCTTGCGCAGTTT	CGTCTAGCAAACTGGAGCAA	40.0	50.0	57.8	58.2	1401 bp	0.5	56.8