Hotspot

groEL-ycf65

Genome · 1588-2625 · 1038 bp

Back to Chroomonas

Pi 0.456

MarkerSeek score 80.90

Primer available yes

Label groEL-ycf65

Feature Metadata

label_name: groEL-ycf65
feature_type: igs
region: Genome
length_bp: 1038
variable_sites: 404
pi: 0.456
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
groEL-ycf65_p1	TGCGACTAAAGCAGCTGTAG	TCCCCAAACGAATGAAAGCA	50.0	45.0	58.0	58.0	1868 bp	0.5	59.9
groEL-ycf65_p2	CGCGTTCTGGACTTCAAAAC	TCCCCAAACGAATGAAAGCA	50.0	45.0	58.0	58.0	1566 bp	0.5	59.8
groEL-ycf65_p3	CGTTCTGGACTTCAAAACGC	TCCCCAAACGAATGAAAGCA	50.0	45.0	58.0	58.0	1564 bp	0.5	59.8
groEL-ycf65_p4	GCGTTCTGGACTTCAAAACG	TCCCCAAACGAATGAAAGCA	50.0	45.0	58.0	58.0	1565 bp	0.5	59.8
groEL-ycf65_p5	GGACTTCAAAACGCAGCTTC	TCCCCAAACGAATGAAAGCA	50.0	45.0	58.0	58.0	1558 bp	0.5	59.6