Hotspot

trnN-GUU-rpl21

SSC · 105709-108727 · 3019 bp

Back to Christella

Pi 0.0162

MarkerSeek score 61.30

Primer available yes

Label trnN-GUU-rpl21

Feature Metadata

label_name: trnN-GUU-rpl21
feature_type: igs
region: SSC
length_bp: 3019
variable_sites: 49
pi: 0.0162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl21_p1	ATGGGGATGGGGAAGATGAA	GCAGCTTCTACTACAGCAGG	50.0	55.0	58.1	58.1	1008 bp	1.0	80.8
trnN-GUU-rpl21_p2	AAAGAGATGGGGATGGGGAA	GCAGCTTCTACTACAGCAGG	50.0	55.0	58.0	58.1	1014 bp	0.5	59.5
trnN-GUU-rpl21_p3	AAAGAGATGGGGATGGGGAA	TTCTTTGTGTGGCATACCCC	50.0	50.0	58.0	58.1	1105 bp	0.5	59.3
trnN-GUU-rpl21_p4	TGGACCAGCCTCTTAAGGAA	GCAGCTTCTACTACAGCAGG	50.0	55.0	58.0	58.1	676 bp	0.5	59.2
trnN-GUU-rpl21_p5	TGGACCAGCCTCTTAAGGAA	TTCTTTGTGTGGCATACCCC	50.0	50.0	58.0	58.1	767 bp	0.5	59.0